Bentonite Flocculation (BF) Method Evaluation comparing to Elisa, Indirect Immunofluorescence (IFA) and Latex Agglutination (LA) in Diagnosis of Toxoplasmosis

Toxoplasmosis is a common disease among human and livestock with a worldwide distribution. This disease will be generated by a unicellular parasite called toxoplasmosis Gondii. The disease usually is diagnosed in laboratory through several methods such as; ELISA, Immunofluorescence and Chemo luminescence. The mentioned methods have sufficient sensitivity and trait, but since they are need to use expensive equipment’s, mostly a lot of time and noticeable costs, the flocculation bentonite can be considered as a screening way to quickly and efficiently diagnosis this disease depends in case it has acceptable sensitivity, trait and efficiency. In this paper as a descriptive/ analytical research, 127 patrons who referred to two reliable medical Labs.in Tehran, so 127 suspected samples were collected and evaluated by using of ELISA, IFA, LAT and BFA methods. Also, in the present paper we measured the ability and efficiency of flocculation bentonite way to evaluate the epidemiologic parameters. The results showed that comparing to other methods such as; ELISA, immunofluorescence and latex agglutination, the flocculation bentonite has 100% sensitivity, 85.71% trait and 90.95% efficiency. Hence, as for the almost good sensitivity, trait and efficiency rate of flocculation bentonite, we can use this system to evaluate and take a significant population under study in respect of spread and indication of toxoplasmosis disease and screening its positive cases, then in order to avoid wasting high time and cost, we can prove the result by using other methods such as immunofluorescence and ELISA.